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101.
近年来,分子标记辅助育种取得了较大进展,各种标记方法的广泛使用极大地加快了新品种的选育速度。随机引物扩增DNA多态性分子标记(RAPD)因其灵敏度高、速度快,可对整个基因组进行标记且费用和技术难度较低,易于应用而倍受青睐[1],已在植物亲缘关系与品种鉴定[2~4]、遗传多样性检测[5,6]、遗传图谱的构建与基因定位[7]、辅助选择育种[8,9]、育种材料的早期的选择、突变体的鉴定等方面得到了较多的应用[10~13]。由于突变体是植物基因组(达2 0×109bp)少数位点发生突变的结果,1个随机引物仅能检测基因组的极少部分,因而突变体的检测相对困… 相似文献
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The relationship of branch cross sectional area (CS) to leaf biomass (LM) and leaf area (LA) was studied in three agroforestry tree species,Calliandra calothyrsus Maissn.,Erythrina berteroana Urban andErythrina poeppigiana (Walpers) O.F. Cook, to develop a non-destructive method for the estimation of LM and LA for trees managed with periodic pruning. Variation in these relationships was observed according to the bifurcation level and, in theErythrina spp., by clone. All the relationships were linear except the CS-LM relation in small branches ofE. poeppigiana, where it was initially exponential. At main branch level the relationship of CS to LM and LA was linear in all cases but the regression parameter values varied between species and clones, with determination coefficient (R2) 0.88–0.99. It was concluded that the ratio of main branch CS to LM and LA can be used for non-destructive estimation of the latter variables. The method has the additional benefit that the regression parameter value reflects the allocation of dry matter within a tree and, consequently, may give indications about its possible uses in different agroforestry systems.Work carried out at the Centro Agronómico Tropical de Investigación y Enseñanza (CATIE), Turrialba, Costa Rica. 相似文献
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桉树E uca lyp tus为世界热带、亚热带地区的极有价值的造林树种.对邓恩桉在湘中地区引种后的生长情况进行调查.结果表明:在桉树引种北缘区,邓恩桉年平均高生长为2.3~3.0 m,胸径生长2.2~3.0 cm,表现出一定的抗瘠薄能力、抗寒耐寒性和速生性.扼要地总结了邓恩桉种苗繁殖技术的进展,并就存在的问题提出了解决方法. 相似文献
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Open pollinated progenies from 15 clones of Japanese black pine (Pinus thunbergii), that had been selected for their tolerance to pine wilt disease, were tested by artificial inoculation with pine wood nematode
for six years at a nursery of Kyushu Regional Breeding Office. Family variations in the respective year were all significant.
An analysis of variance across the six years’ tests confirmed highly significant effect of year and of families; however,
the year by family interaction was significant also. Phenotypic and genetic variances estimated from each year’s result were
fitted to a quadratic regression using their mean survival ratio as an independent variable. The variances were greatest at
around 0.5 of the mean survival ratio. Estimated heritability showed a similar trend of change, whereas the peak of the expected
amount of improvement shifted toward the lower survival range: around 0.4, where a greater selection differential was anticipated.
It was concluded that a useful amount of genetic improvement that may be reduced by 30% due to the interaction, would be expected
by prescreening seedlings where the mean survival ratio after inoculation was around 0.25 to 0.6. 相似文献